Figure 3

Inhibition of MAPkinases activation by PD98059, SB203580, SP600125. HAM were preincubated during 1 h with inhibitors (50 μM) and then stimulated 30 min with LPS (1 μg/ml). Levels of phosphorylated ERK, p38 and JNK MAP kinases were evaluated by Western-blot analysis. Each bands was normalised by performing phospho MAP kinase to β-actin ratio as by the graph representing the densitometric analysis.