Figure 2

Activation of MAP kinases in HAM stimulated by LPS. Panel A: HAM were stimulated 30 min with increasing doses of LPS and phosphorylated ERK, p38 and JNK were detected by Western-blotting as described in Materials and Methods. Panel B: HAM were incubated with LPS (1 μg/ml) during different time and phosphorylated ERK, p38 and JNK were detected by Western-blotting. Below the blots, the graph represents the phosphorylated MAP kinases to β-actin ratio obtained by densitometric analysis of each bands using Quantity One Sotware.