|
Parameter
|
D0
|
D3
|
D6
|
D9
|
WT
|
|---|
|
V(lung) [cm
3
]
|
1.00 (0.06)
|
0.72 (0.02)†
|
0.82 (0.07)†
|
0.76 (0.1)†
|
0.68 (0.22)†
|
|
N(alv,lung) [10
6
]
|
5.80 (0.52)
|
7.03 (0.58)†
|
8.51 (1.01)†
|
7.36 (0.58)†
|
8.75 (3.18)
|
|
N
V
(alv/par) [10
3
/mm
3
]
|
6.65 (0.58)
|
10.71 (0.51)†
|
11.17 (0.92)†
|
10.81 (0.95)†
|
14.11 (0.76)†||§**
|
N
(alv) [10
3
μm
3
]
|
139.67 (6.87)
|
79.26 (5.78)†
|
75.66 (5.1)†
|
82.07 (8.19)†
|
59.40 (9.19)†||§**
|
|
S(alvepi,lung) [cm
2
]
|
597.7 (51.9)
|
583.3 (66.4)
|
671.5 (74.1)
|
592.8 (61.2)
|
575.3 (157.2)
|
|
S
V
(alvepi/par) [1/cm]
|
661.8 (46.3)
|
910.3 (65.7)†
|
881.1 (47.1)†
|
866.6 (38.0)†
|
959.5 (146.3)†
|
|
V
V
(air/par) [%]
|
90.4 (2.3)
|
86.8 (3.1)
|
84.2 (3.0)†
|
88.2 (4.1)
|
83.5 (11.1)
|
|
V
V
(sep/par) [%]
|
10.4 (0.5)
|
13.8 (1.7)†
|
13.6 (1.5)†
|
13.4 (1.8)†
|
17.3 (7.2)†
|
 (sep) [μm]
|
3.14 (0.22)
|
3.04 (0.58)
|
3.08 (0.46)
|
3.08 (0.4)
|
3.5 (0.86)
|
|
N(typeII,lung) [10
6
]
|
12.57 (1.61)
|
12.58 (0.76)
|
11.02 (0.64)†
|
7.29 (0.97)†||§
|
7.73 (1.91)†||§
|
|
N
V
(typeII/par) [10
3
/mm
3
]
|
13.87 (1.12)
|
19.74 (1.58)†
|
14.52 (0.62)||
|
10.6 (0.59)†||§
|
13.15 (3.54)||
|
|
N
(typeII) [μm
3
]
|
467.5 (18.6)
|
408.97 (36.1)†
|
417.15 (17.5)†
|
418.38 (10.9)†
|
348.5 (28.53)†||§**
|
|
V
V
(lb/typeII) [%]
|
24.7 (2.5)
|
23.5 (4.4)
|
19.7 (1.9)†
|
19.8 (2.1)†
|
15.3 (1)†||§**
|
|
V(lb,typeII) [μm
3
]
|
114.9 (8.3)
|
97.0 (25.7)
|
82.1 (8.7)†
|
82.7 (10.7)†
|
53.2 (6.2)†||§**
|
|
V(lb,lung) [mm
3
]
|
1.44 (0.19)
|
1.21 (0.31)
|
0.9 (0.11)†
|
0.61 (0.14)†||§
|
0.42 (0.14)†||§
|
|
V
(lb) [μm
3
]
|
0.93 (0.12)
|
1.40 (0.35)
|
0.67 (0.08)†||
|
0.78 (0.03)||
|
0.48 (0.08)†||§**
|
- Summarized stereological data, grouped into parameters related to parenchymal architecture, to type II cells, and to lamellar bodies. Values are given as mean (SD) of n = 4–5 mice per group. Abbreviations: V = volume, VV = volume density, S = surface area, SV = surface area density, = mean thickness, N = number, NV = numerical density,
N = number-weighted mean volume,
V = volume-weighted mean volume, par = parenchyma, air = airspace, sep = septal tissue, alvepi = alveolar epithelium, alv = alveoli, typeII = type II cells, lb = lamellar bodies. Statistically significant differences between groups are indicated as: † p < 0.05 vs. group D0, || p < 0.05 vs. group D3, § p < 0.05 vs. group D6, ** p < 0.05 vs. group D9.
N
(alv) [10
3
μm
3
]
(sep) [μm]