Salmeterol and cytokines modulate inositol-phosphate signalling in Human airway smooth muscle cells via regulation at the receptor locus

Table 2 Effect of pre-treatment with drugs or cytokines on the magnitude of Human ASM IPx signalling responses following histamine, bradykinin or sodium fluoride stimulation.

Cytokine/drug	Histamine response	Bradykinin response	NaF response
IL-1β	97.0 (+/- 4.1)	97.8 (+/- 4.3)	85.9 (+/- 8.1)
TNFα	113.0 (+/- 8.4)	127.4 (+/- 8.3) +**	102.8 (+/- 11.9)
IL-13	144.3 (+/- 9.3) +**	113.8 (+/- 7.0)	124.2 (+/- 15.6)
IFNγ	126.4 (+/- 7.5) **	128.0 (+/- 8.4) +**	132.3 (+/- 14.0)
Salmeterol	117.7 (+/- 5.2) **	111.7 (+/- 5.0) *	116.2 (+/- 10.9)
Dexamethasone	105.6 (+/- 4.4)	100.3 (+/- 5.0)	113.2 (+/- 10.4)

ASM cells were serum starved for 24 hours and then treated with medium alone, 10 ng/ml IL-1β, 10 ng/ml TNFα, 10 ng/ml IFNγ, 50 ng/ml IL-13, 1 μM dexamethasone or 1 μM salmeterol for 24 hours. Cells were loaded with [³H]-myo-inositol and IPx production following 100 μM Histamine, 0.1 μM bradykinin or 10 mM Sodium Fluoride stimulation was determined as described [30]. The IPx production in the medium alone treated cells was normalised to 100% and the other treatment groups determined relative to this. Data represents the mean response +/- S.E.M, n = 9 (three independent experiments for 3 donors). Data within groups was compared by ANOVA and post hoc Dunnett's Multiple Copmarison Test (+p < 0.05) or T-Test (*p < 0.05, **p < 0.01).

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