Salmeterol and cytokines modulate inositol-phosphate signalling in Human airway smooth muscle cells via regulation at the receptor locus

Table 1 Primers and probes

	REAL TIME PCR
GENE	5'PRIMER	3'PRIMER	PROBE (5'FAM/3'TAMRA)	LOCATION
HRH1	TCTCGGTGGCGGACTTGA	CATGAGCAGGTAGAGGATGTTCAT	CGTGGGTGCCGTCGT	ORF
BDKRB2	GATCAGCACCTTCCTGGATACG	GATGATGCGCTCGTCCTGG	CATCGCCTCGGCATCCTCTCCA	ORF
GNA11	TCAGCGAATACGACCAAGTCC	AGGGCTTTGCTCTCCTCCA	TGGAGTCGGACAACGAGAACCGG	Exon 5/6 boundry
PLCB1	CTGCCTGCTGTCTTTGTCTACATAG	TCGGATTGGGTTTGATAAAGCT	TGAAAGACTATGTGCCAGACACA TATGCAGATG	Exon 22/23 boundary
	5'RACE PRIMER 1 (PCR 1)	PRIMER 2 (PCR 2)
BDKRB2	CACGAACAGCACCCAGAGGAAGG	CCAGCCCAGCCACTCCACTTG
	PLASMID CONSTRUCTION 5'PRIMER	3'PRIMER	LOCATION IN CONTIG	CONSTRUCT
HRH1-1	CTGACTCGAGCCTCCTCTCCCTGTGAGCTT	CTGAAAGCTTGCCGGCTCCGGGGAAAGTT	51,750 – 52,761	HRH1 1 kb
HRH1-2	CTGACTCGAGGAAAGTGTGGACGCAGCCATT	CTGAAAGCTTGCCGGCTCCGGGGAAAGTT	50,782 – 52,761	HRH1 2 kb
HRH1-3	CTGACTCGAGGTTCAATCATAGCTCACTGCAG	CTGAAAGCTTGCCGGCTCCGGGGAAAGTT	49,768 – 52,761	HRH1 3 kb
HRH1-4	CTGACTCGAGTCCACAGCTTGTTGCACAGC	CTGAAAGCTTGCCGGCTCCGGGGAAAGTT	48,718 – 52,761	HRH1 4 kb
BDKRB2-1	CTGACTCGAGCCCAGGCCACCCATAAACTG	CTGAAAGCTTCAAGCGGCATGGGCACTTCA	101,405 – 102,439	BDKRB2 1 kb
BDKRB2-2	CTGACTCGAGGGGCGGATGTGTGGGTAGAT	CTGAAAGCTTCAAGCGGCATGGGCACTTCA	100,436 – 102,439	BDKRB2 2 kb
BDKRB2-3	CTGACTCGAGCCTCCTATGTGCCGGGTGAT	CTGAAAGCTTCAAGCGGCATGGGCACTTCA	99,416 – 102,439	BDKRB2 3 kb
BDKRB2-4	CTGACTCGAGATGTTGGCCAGGCTGATCTC	CTGAAAGCTTCAAGCGGCATGGGCACTTCA	98,414 – 102,439	BDKRB2 4 kb

*HRH1 reference contig = AC083855, BDKRB2 reference contig = AL355102.5. Bold denotes HindIII and XhoI restriction sites.

ISSN: 1465-993X