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Figure 5 | Respiratory Research

Figure 5

From: Salmeterol and cytokines modulate inositol-phosphate signalling in Human airway smooth muscle cells via regulation at the receptor locus

Figure 5

Effect of salmeterol or cytokine treatment for 24 hours on HRH1 promoter activity in Human ASM cells transfected with HRH1-Luciferase or control constructs. Human ASM cells were transfected and stimulated as described in Figure 4. Following 24 hours treatment cells were harvested and firefly luciferase was quantified. pGL4-Luc2 (A), pGL4-SV40-Luc2 (B), pGL4-HRH1-1 kb-Luc2 (C), pGL4-HRH1-2 kb-Luc2 (D), pGL4-HRH1-3 kb-Luc2 (E), pGL4-HRH1-4 kb-Luc2 (F) transfections. Data is normalised to the mean luciferase activity of each construct transfection treated with medium alone +/- S.E.M. (n = 5 independent experiments) Dunnett's Multiple Copmarison Test (**p < 0.01).

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Respiratory Research

ISSN: 1465-993X

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