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Table 1 Real-time PCR primers description

From: Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema

Gene GenBank accession number Primer sequences Amplicon size Annealing temperature
18S rRNA NR_003286 F: 5'-TGCATGGCCGTTCTTAGTTG-3' 76 bp 57°C
   R: 5'-AGTTAGCATGCCAGAGTCTCGTT-3'   
Perforin A26435 F: 5'-CGCCTACCTCAGGCTTATCTC-3' 155 bp 58°C
   R: 5'-CCTCGACAGTCAGGCAGTC-3'   
Granzyme B NM_004131 F: 5'-TGGGGGACCCAGAGATTAAAA-3' 100 bp 55°C
   R: 5'-TTTCGTCCATAGGAGACAATGC-3'   
FasL AF288573 F: 5'-TCAATGAAACTGGGCTGTACTTT-3' 101 bp 58°C
   R: 5'-AGAGTTCCTCATGTAGACCTTGT-3'