Blood inflammatory cells counts and CD4/CD8 ratio. A) Distribution of systemic inflammatory cells in the three groups obtained by automatized cell count. B) PBMC were isolated from each subjects, double labelled with fluorochrome-conjugated antibodies (CD3/CD4 and CD3/CD8), and analysed by flow cytometry. For each subject, CD4/CD8 ratio was obtained by dividing the %CD3+/CD4+ cells by the %CD3+/CD8+ cells. Columns represent mean ± SEM. *p = 0.02. Normal non-smokers (white), normal smokers (black), and emphysematous smokers (grey).