Figure 1

Effect of exogenous VEGF and anti-VEGF antibody on LPS-induced lung injury. a) T/P ratio 6 h after intratracheal LPS instillation. Mice received treatment with either PBS, VEGF or anti-VEGF Ab intravenously. The group with LPS challenge revealed increased T/P ratio (*p < 0.0001). The T/P ratio of LPS+anti VEGF Ab group tends to increase compared with that of LPS group. In the LPS+VEGF (pre- and post-treatment) groups, T/P ratio was significantly decreased compared with LPS group (^†p < 0.01, ^‡p < 0.05, respectively). n = 6 in each group. b) W/D ratio 6 h after intratracheal LPS instillation. Mice received treatment with either PBS, VEGF or anti-VEGF Ab intravenously. The group with LPS challenge revealed increased W/D ratio (*p < 0.01). The W/D ratio of LPS+anti VEGF Ab was not significantly different from that of the LPS group. In the LPS+VEGF (Pre) group, W/D ratio was significantly decreased compared with the LPS group (^†p < 0.05). n = 6 in each group. c) I/T ratio 6 h after intratracheal LPS instillation. The group with LPS challenge revealed increased I/T ratio (*p < 0.0001) compared with the control group. In the anti-VEGF Ab group, I/T ratio was greater than LPS group (^†p < 0.05). In the LPS+VEGF (pre) group, I/T ratio was significantly decreased compared with LPS group (^‡p < 0.0001). n = 6 in each group. d) Emigrated neutrophils 6 h after intratracheal LPS instillation. Mice received treatment with either PBS, VEGF or anti-VEGF Ab intravenously. The LPS group revealed an increase in emigrated neutrophil (*p < 0.05). Treatment with anti VEGF Ab significantly enhanced neutrophil emigration, compared with the LPS group (^†p < 0.05). Pre- or post-treatment with VEGF did not affect neutrophil emigration, compared with the control and the LPS groups. n = 6 in each group.