Figure 5

TGFα and ADAM17 expression patterns are consistent with IL-13-induced movement of TGFα. Confocal microscopy was used to determine the cellular distribution of TGFα and ADAM17 in NHBE cells following stimulation with IL-13 for various lengths of time. Representative images from cultures of NHBE cells treated with media only (control) or IL-13 (10 ng/ml) for 15 or 30 min are shown. NHBE cultures were imaged in Z-stack mode from the basal to the apical boundaries of the cells. Images shown are x-y planes (large squares) halfway between the basal-most and the apical-most images, bordered by corresponding y-z planes (shown at right of x-y plane) and x-z planes (shown at bottom of x-y plane). The y-z and x-z plane images are from the sites indicated by the white arrows at the bottom and the right of the x-y plane images, respectively. a → b denotes the apical (a) to basal (b) direction as it relates to the x-z and y-z planes. TGFα (red) and ADAM17 (green); scale bars represent 10 μm.