Figure 4
IL-13-induced effects are not due solely to activation of ADAM17. a) NHBE cells were exposed to IL-13 (10 ng/ml) or control media for 4 or 24 hrs, and steady-state mRNA levels of ADAM17 and β-actin determined via RT-PCR. Ethidium bromide-stained gels of PCR products are shown. b) NHBE cells were treated with control media or IL-13 for the times indicated. Total protein from these cells was examined for ADAM17 expression via Western blot. Membranes were chemically stripped and rehybridized to detect β-actin as a control for equal protein loading. c) NHBE cells were treated with control media, IL-13, or PMA (10 nM) for 1 hr and the supernatants examined for soluble TGFα via ELISA (n = 4, *p < 0.05 compared to control). d) NHBE cells were treated for 24 hrs with control media, IL-13, or PMA (10 nM), and [3H]-thymidine incorporation determined as a measure of proliferation (n = 6, *p < 0.05 compared to control). e) Secretion of IL-8 from NHBE cells was examined by ELISA following 1 hr exposure to control media, IL-13, or PMA (10 nM) (n = 6, *p < 0.05 compared to control).