Figure 2

Inhibitors of ADAM17 attenuate IL-13-induced shedding of TGFα. NHBE cells were exposed to control media, inhibitors of ADAM17, IL-13 or IL-13 plus inhibitors for 1 hr. a) NHBE cells were exposed to either control media (no inhibitor), TIMP-1 or TIMP-3 (both at 2 μg/ml) for 30 min prior to treatment with IL-13 (10 ng/ml) or control media. The inhibitors were also included during the treatment period. After the 1 hr treatment, supernatants were examined for TGFα shedding via ELISA (n = 4, *p < 0.05 vs. corresponding control, †p < 0.05 vs. IL-13 alone). Light gray bars = TIMP-1; Dark gray bars = TIMP-3. b) NHBE cells were exposed to control media, anti-ADAM17 antibodies, IL-13, or IL-13 plus anti-ADAM17 for 1 hr. Supernatants were then examined for shed TGFα via ELISA (n = 6, *p < 0.05 vs. media control, †p < 0.05 vs. IL-13 alone).