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Table 1 Specificity of purified CD8+ CTL a

From: Control of mucosal virus infection by influenza nucleoprotein-specific CD8+ cytotoxic T lymphocytes

     % specific lysis b
Stimulation Cell Fraction % CD8+ cells % CD4+ cells A/HK/68 Vac-NP Vac-gp120 B/Panama
Vac-NP CD8+ 97.3 1.1 22.4 44.3 1.3 0.0
  CD8- 0.6 75.5 NT 1.1 NT 2.7
Vac-gp120 CD8+ 90.1 0.0 0.34 0.0 9.02 NT
  CD8- 0.0 74.2 NT 3.3 NT NT
  1. a Splenocytes from Balb/c mice primed with influenza A/Taiwan (H1N1) virus were stimulated with 2 pfu/cell of recombinant Vac-NP or vaccinia-gp120 for 5 days. CD8+ T cells were obtained by positive selection using AutoMacs instrument. The percentage of CD8+ and CD4+ T cells determined by dual color flow cytometry and % lysis in purified CD8+ and CD8- fractions are presented. b Lysis of P815 (H-2d) target cells with the indicated infection was determined in the 4-hour chromium release assay. Percent specific lysis at 40:1 E:T ratio is shown. NT = not tested.