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Table 2 Primer and probe sequences for murine mucins

From: Dietary n-3 fatty acids have suppressive effects on mucin upregulation in mice infected with Pseudomonas aeruginosa

Mucin

 

Sequence (5'-3')

Product length

Muc4

FW

GTCCACTTCTTCCCCATCTC

 
 

RV

GTAGCCTTTGTAGCCATCACAT

 
 

PB

CCAGGACCAGATGGCTCTGAACCT

102 bp

Muc5b

FW

GCACGTAAATGCGACTGTCT

 
 

RV

ATGGACCTTGCTCTCCTGAC

 
 

PB

TATCCAAGTACTCCATGGAGGCCC

133 bp

Muc5ac

FW

TCCCTTACCTAACCAGCAGAA

 
 

RV

GGGAGTACATGGAGATGCTGT

 
 

PB

GAGGGCCCAGTGAGCATCTCCTACT

118 bp

  1. FW, forward primer; RV, reverse primer; PB, TaqMan probe dual-labeled with 5'FAM and 3'TAMRA.
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Respiratory Research

ISSN: 1465-993X

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