Figure 8

Desipramine and fluoxetine inhibit RANTES expression. A, A549 cells were left untreated (UT) or stimulated for 20 h with TNF-α at 10 ng/ml. Concentration of RANTES in supernatants was then measured. *p < 0.0001 versus untreated, by Welch t test. B, A549 cells were stimulated for 20 h with 10 ng/ml of TNF-α in the presence of increasing concentrations of desipramine or fluoxetine. Data are shown as the percentage of TNF-α-induced RANTES release. *p < 0.05 versus TNF-α alone, by ANOVA with Bonferroni post-test. C, A549 cells were either left untreated (UT) or pretreated or not for 1 h with desipramine (DMI) or fluoxetine (Fluo) at 10^-5 M and further stimulated for 4 h with TNF-α at 10 ng/ml. The amount of RANTES mRNA was quantified by a colorimetric assay. *p < 0.05 versus TNF-α alone, by Student t test.