Figure 4

Validation of selected genes by Taqman quantitative RT-PCR. RNA was isolated from human lung fibroblasts treated with 10 μg/cm² V₂O₅ at the indicated time points and RT-PCR performed as described in Methods. Three genes from four categories were validated; growth factors (top row: VEGF, HBEGF, CTGF), chemokines (second row: IL8, CXCL9, CXCL10), transcription factors (third row: Egr1, STAT1, GAS1), and oxidative stress genes (bottom row: PIPOX, OXR1, SOD2). The data for each gene was normalized against 18S housekeeping gene and expressed as the mean ratio. Data are representative of at least two replicate experiments and expressed as the mean ± sem of triplicate dishes of cells. The temporal pattern of each V₂O₅-altered gene validated by RT-PCR is compared with the result obtained from the microarray experiment (open diamonds).