Figure 5

Effects of inhibitors of EGFR tyrosine kinase and Src tyrosine kinase on PAR ₄ agonist-induced changes in EMT-related marker protein expression in A549 cells. A549 cells were pretreatd with or without 30 nM AG1478 or 300 nM PP2 for 30 min, and then stimulated with AYPGKF-NH₂ (100 μM) for 96 h followed by immunoblotting with specific antibodies for E-cadherin, α-SMA. (A, B) Representative Western blots for the effect of AG1478 (A) or PP2 (B) on the expression of E-cadherin and α-SMA. (C, D) Summarized densitometric data. Each bar represents the mean ± S.E.M. for 4–12 preparations. *P < 0.05 compared with AYPGKF-NH₂ alone.