Figure 5

Effects of NOR-1 on PKC isoforms expression in A549 cells. Cell extracts were portioned into cytosol (C) and membrane (M) fractions as described under "Materials and Methods." PKC isoforms were detected by Western blotting. NOR-1 (0.5 mM) induced migration of PKCα and PKCδ but not PKCγ and PKCε from the cytosol to the membrane. PKC-α,β inhibitors, GÖ6976 (10 nM) and PKCδ inhibitors, rottlerin (4 μM) inhibited the NOR-1 induced migration of PKCα and PKCδ respectively. NOR-1 induced migration of PKCα and PKCδ were also inhibited by calphostin C (0.5 μM).