Figure 4

Effects of NO donor and PMA on the distribution of PKCα in A549 cells. A549 cells were exposed to NOR-1 (0.5 mM) or PMA (1 μM) for one hour and then fractionated. Proteins of equal amounts were separated by SDS-PAGE, transferred, incubated with anti-PKCα antibodies, and detected using a chemiluminescence method. The results were expressed as means ± SE of three independent experiments. * p < 0.01 versus control membrane. † p < 0.01 versus control cytosol.