Figure 1From: Antagonism of the prostaglandin D2 receptor CRTH2 attenuates asthma pathology in mouse eosinophilic airway inflammationIn vitro characterization of compounds on mouse CRTH2 (mCRTH2) and mouse TP (mTP) receptors. A, Competition binding analysis. B, inhibition of mCRTH2 and mTP receptor function. PGD2- or U-46619-concentration response curves in the absence and presence of the indicated compounds in mCRTH2- or mTP-expressing cells. Inserts: Schild plots. Schild analysis show potent antagonism of mCRTH2 by TM30089 (pA2 = 9.15 ± 0.11, Schild slope = 1.45 ± 0.08) whereas it does not interfere with signaling of mTP. Ramatroban is a potent antagonist on mCRTH2 (pA2 = 8.08 ± 0.14, schild slope = 0.94 ± 0.05) and mTP (pA2 = 9.36 ± 0.10, Schild slope = 1.35 ± 0.06). Experiments show mean ± SE from 5–7 independent experiments.Back to article page