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Figure 3 | Respiratory Research

Figure 3

From: Upregulation of pirin expression by chronic cigarette smoking is associated with bronchial epithelial cell apoptosis

Figure 3

Mean RNA expression levels for pirin assessed by quantitative PCR in the human bronchial epithelial (BEAS-2B) cell line infected with AdPirin, AdNull, and negative control (naive) over varying concentrations (particle units, pu) and time. RNA was extracted from BEAS-2B cells that were infected with 103 and 104 particle units of AdPirin or AdNull. A. Northern analysis for Pirin RNA demonstrating the full length (1.1 kb) pirin mRNA directed by AdPirin 24 hr after infection. Lane 1 – control (naive); lane 2 – AdNull, 103 particle units; lane3 – AdNull, 104 particle units; lane 4 – AdPirin, 103 particle units; and lane 5 – AdPirin, 104 particle units. B. Concentration-dependent increase in pirin mRNA following infection with AdPirin. Pirin mRNA was quantified by TaqMan analysis. C. Time-dependent pirin mRNA expression over time evaluated by TaqMan.

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