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Figure 5 | Respiratory Research

Figure 5

From: Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an α-smooth muscle actin-Cre transgenic mouse

Figure 5

Phenotypic analysis of the human bronchial epithelial cell line (16HBE) following exposure to TGF-β1. Immunofluorescent staining for E-cadherin (a, b) showed that exposure to TGF-β1 (b) resulted in an apparent reduction and redistribution of E-cadherin from intercellular junction areas into cytoplasm, compared to control (a). Mesenchymal marker F-actin, was faintly stained at the cell margin in the control (c), whereas the staining was substantially enhanced and abundantly located throughout cytoplasm after TGF-β1 stimulation (d). Immunofluorescent staining for (-SMA was not detected in the cells under basal conditions (e), but was observable in a few cells after TGF-β1 exposure (f).

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