Table 1 Antibodies utilised in this study
From: Migratory marker expression in fibroblast foci of idiopathic pulmonary fibrosis
Antigen | Clone/Ab | Reactivity | Source |
|---|---|---|---|
heat shock protein 27 | clone 2B4 | FF* in UIP | Novocastra |
Heat shock protein 27, phosphorylated on serine-82 | S82, rabbit monoclonal | FF in UIP | Epitomics-Inc, |
laminin-5γ-2 chain | 4G1 | FF in UIP | DakoCytomation |
fascin | 55K-2 | FF in UIP, vessels, myofibroblasts, dendritic cells | DakoCytomation |
α-smooth muscle actin | 1A4 | smooth muscle, myofibroblasts in FF | DakoCytomation |
CC10 | urine protein 1 (rabbit polyclonal) | Clara cells | DakoCytomation |
cytokeratin 8/18 | clone 5D3 | epithelial cells | Biogenex |
cytokeratin 5 | clone XM26 | bronchiolar basal cells | Novocastra |
ΔN-p63 truncated isoform | p40 (rabbit polyclonal) | bronchiolar basal cells | Oncogene Research |
surfactant-apoprotein A (SP-A) | clone PE-10 | pneumocytes | DakoCytomation |
tenascin | TN2 | FF in UIP | DakoCytomation |