Figure 2

Characterisation on serial sections of the cells expressing LAM5γ2, fascin and HSP27 in "sandwich-FF" of UIP biopsies. (a) A fibroblast focus is shown by tenascin immunostaining in a UIP biopsy at the edge between dense scarring and scarcely involved lung (square frame). In (b) The same FF is analysed for HSP27 (brown) and tenascin (red) by double-marker immunostaining. The same lesion was studied using serial sections, showing that surfactant SPA is not expressed by overlying epithelial cells (c), but a cluster of basal cells expresses high level of fascin (d) and MMP7/matrylisin (e). In dmyofibroblasts show discrete immunoreactivity for fascin. In the f-g-hsequence a "sandwich-FF" is shown, immunostained on serial sections for fascin (f), LAM5γ2 (g), and keratin-5, a marker of bronchiolar basal cells (h).