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Figure 7 | Respiratory Research

Figure 7

From: Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells

Figure 7

Inhibitory effect of cAMP elevating agents on MMP-9 activity. ELISA data of total MMP-9 activity in conditioned medium after 48 h, showing stimulation following treatment of PASMCs with PMA (10-7 M) and TNF-α (10 ng/ml) and its inhibition by cicaprost and forskolin (A-B). Inhibitory effect of roflumilast, both alone (C) and in combination with a sub-maximal concentration of cicaprost (D). Data represent mean ± SEM of four replicates (A-B) and three-four distinct PASMC isolates (C-D). * P < 0.05, ** P < 0.01 and *** P < 0.001 versus medium from control cells in serum free (SF) medium (A) or PMA and TNF-α treatment (B-D).

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