Figure 5
Pro-apoptotic effects of cAMP elevating agents. Concentration-dependent effect of iloprost on apoptosis, as demonstrated by the proportion of Hoechst-stained cells showing characteristic condensed nuclear fluorescence (A) and measurement of DNA fragmentation in human PASMCs (B). Effects of PDE4 inhibition (10-6 M roflumilast) and adenylyl cyclase activation (10-5 M forskolin) on DNA fragmentation (C). Data represent mean ± SEM of four replicates in three distinct isolates. * P < 0.05, ** P < 0.01 and *** P < 0.001 versus untreated control cells in serum free (SF) medium.