Figure 2From: Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cellsCharacterisation of cAMP phosphodiesterase (PDE) activity in human PASMCs. Total cAMP hydrolytic activity and contribution of PDE enzyme families to cAMP hydrolysis in the cytosol (A) and membrane fractions (B) of human PASMCs (n = 5 isolates). Activity inhibited by 10-3} M EGTA (PDE1), 10-5 M EHNA (PDE2), 10-5 M cilostamide (PDE3) and 10-6 M roflumilast (PDE4).Back to article page