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Figure 4 | Respiratory Research

Figure 4

From: RhoA signaling modulates cyclin D1 expression in human lung fibroblasts; implications for idiopathic pulmonary fibrosis

Figure 4

Cell proliferation is enhanced in IPF lung fibroblasts and can be abrogated by RhoA inhibition. 4a. Cell proliferation was determined by incorporation of BrdU in three separate human lung fibroblast cell lines from IPF patients (HIPF, LL29, and LL97a) and normal control equivalents CCD8LU data represents analysis in the IPF lung fibroblast cell line LL97a. Cells were subjected to BrdU incorporation at 24-hour intervals as described. Data are representative of the mean of 3 independent experiments (standard error bars have been omitted to simplify the figure). * = p < 0.05 significant elevation relative to serum free controls. 4b. Cell proliferation in response to the recognised Rho inhibitor C3 exotoxin (0.5–5 μg/ml) with or without subsequent TGF-β1 (5 ng/ml) stimulation was measured by BrdU incorporation in three separate human lung fibroblast cell lines from IPF patients (HIPF, LL29, and LL97a) and normal control equivalents CCD8LU. Data shows analysis in the IPF cell line LL97a. Data are representative of the mean of 3 independent experiments (standard error bars have been omitted to simplify the figure). * = p < 0.05 relative to TGF-β1 stimulated cells.

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