Figure 7
Effect of LY294002 and wortmannin on [3H]thymidine incorporation, EGF-R and ERK1/2 phosphorylation induced by LTD 4 or EGF. (A-B) Increase of [3H]thymidine incorporation induced by 1 μM LTD4 and 20 ng/ml EGF alone and in combination in the absence and presence of (A) 50 μM LY294002 or (B) 200 nM wortmannin (30 minutes pretreatment). Control is represented by MEM additioned with 1% FBS. The results are presented as mean ± S.E.M. of at least three experiments performed in triplicate on at least two different cell lines. **P < 0.01 (one-way ANOVA). (C) EGF-R phosphorylation induced by 1 μM LTD4 (3 minutes), in the absence and presence 50 μM LY294002 or 200 nM wortmannin (30 minutes pretreatment). 1 ng/ml EGF was used as an internal control. (D) ERK1/2 phosphorylation induced by 10 nM LTD4 and 0.1 ng/ml EGF (5 minutes) in the absence and presence of 50 μM LY294002 or 200 nM wortmannin (30 minutes pretreatment). The results presented are representative of at least three experiments performed on two different cell lines.