Figure 5
Effect of PTX on [3H]thymidine incorporation, EGF-R and ERK1/2 phosphorylation induced by LTD 4 or EGF. (A) Increase of [3H]thymidine incorporation induced by 1 μM LTD4 and 20 ng/ml EGF alone and in combination in the absence and presence of 100 ng/ml PTX (20 hours pretreatment). Control is represented by MEM additioned with 1% FBS. The results are presented as mean ± S.E.M. of three experiments performed in triplicate on two different cell lines. **P < 0.01 (one-way ANOVA). (B) EGF-R phosphorylation induced by 1 μM LTD4 (3 minutes), in the absence and presence of 100 or 300 ng/ml PTX (20 hours pretreatment). 1 ng/ml EGF was used as an internal control. (C) ERK1/2 phosphorylation induced by 10 nM LTD4 (5 minutes) in the absence and presence of 100 or 300 ng/ml PTX (20 hours pretreatment). The results presented are representative of at least three experiments performed on two different cell lines.