Skip to main content
Figure 2 | Respiratory Research

Figure 2

From: CysLT1 receptor-induced human airway smooth muscle cells proliferation requires ROS generation, EGF receptor transactivation and ERK1/2 phosphorylation

Figure 2

LTD 4 -induced EGF-R phosphorylation. (A) Increase of [3H]thymidine incorporation induced by 1 μM LTD4 and 20 ng/ml EGF alone and in combination, in the absence and presence of 250 nM AG1478 (1 h pretreatment). Control is represented by MEM additioned with 1% FBS. The results are presented as mean ± S.E.M. of three experiments performed in triplicate on two different cell lines. (B) Concentration-response curve of EGF-R phosphorylation induced by 3 minutes treatment with the indicated concentrations of LTD4. (C) Time-course of EGF-R phosphorylation induced by 1 μM LTD4. For B and C 1 ng/ml EGF was used as an internal control and the experiments were repeated twice.

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us