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Table 2 Overview of cell surface expression of several cell surface molecules on rat alveolar macrophages and detection of differential expression in AM from NO2-exposed rats in comparison to AM from untreated controls. Expression analysis was performed by flow cytometry following staining of cells with the respective primary antibody and a signal amplification system.

From: Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats

Antibody

Antigen/Cell population

Expression

Differences

1A29

ICAM-1 (CD54)

medium

medium

1C7

mononuclear phagocytes (CD68 ?)

medium

medium

3.2.3.

NKR-P1 (CD161)

weak

no

3A12

PECAM-1 (CD31)

weak

no

5F10

VCAM-1

no

 

ART18

IL-2 receptor

no

 

ART65

IL-2 receptor

no

 

ED2

macrophage subset (no monocytes)

no

 

ED3

macrophage subset (no monocytes)

no

 

ED4

macrophages

medium

no

ED7

macrophage subset (CD11/CD18; CR3)

medium

strong

ED8

macrophage subset (CD11/CD18; CR3)

medium

small

ED9

macrophage subset (SIRPα, CD172a)

strong

medium

KIM2R

mature tissue macrophages

no

 

MAR3

macrophage subset

no

 

Ox2

CD200

no

 

Ox26

transferrin receptor (CD71)

no

 

Ox3

MHC-II (I-A like)

weak

small

Ox4

MHC-II (I-A like)

weak

small

Ox41

macrophages, DCs, PMNs (SIRP)

no

 

Ox50

hyaluronic acid receptor (CD44)

medium

small

Ox52

activated monocytes

  

Ox6

MHC-II (RT1.B; I-A)

weak

medium

Ox62

DC subpopulation

no

 

Ox8

CD8α

no

 

Ox85

L-selectin (CD62L)

no

 

RM-1

monocytes/macrophages/DCs/PMNs

strong

small

RM-4

all macrophages (no monocytes)

medium

strong

RMA

macrophage subset (120 kDa antigen)

medium

medium

RP-1

neutrophiles (intracellular)

no

 

RP-3

neutrophiles (intracellular)

no

 

W3/13

leukosialin (CD43)

no

 

WT/1

LFA-1 (CD11a)

weak

no