Figure 5From: Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed ratsDifferential cytokine production by LPS-stimulated AM subpopulations of NO2-exposed rats. ED7-positive and ED7-negative AM were separated from 3 days NO2-exposed rats and cultured in vitro for 24 hours in the presence of 100 μg LPS. Subsequently, TNF-α, IL-10, and IL-12 p70 were quantitated in the culture supernatants of ED7-negative (blank bars) and ED7-positive AM (hatched bars) by ELISA. Data are presented as mean ± SD of at least six animals per group. Significance of differences was tested using Students t-test and is indicated by ** for p < 0.01 or *** for p < 0.001.Back to article page