Figure 4
Cytokine and iNOS mRNA expression in AM subpopulations of NO2-exposed rats. ED7-positive and ED7-negative AM were separated from 3 days NO2-exposed rats and total RNA was prepared immediately after cell separation. Cytokine (TNF-α, IL-10, IL-12 p40) and iNOS mRNA expression was analyzed by quantitative RT-PCR with L32 as house-keeping gene control in ED7-negative (blank bars) and ED7-positive AM (hatched bars). Data are presented as relative expression with mean expression in ED7-negative AM was 100 %. Shown are mean ± SD of six animals per group. Significance of differences was tested using the U-test according to Mann and Whitney and is indicated by * for p < 0.05 or ** for p < 0.01.