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Figure 1 | Respiratory Research

Figure 1

From: IL-4 induced MUC4 enhancement in respiratory epithelial cells in vitro is mediated through JAK-3 selective signaling

Figure 1

(a) RT-PCR analysis of IL-4R mRNA expression from NCI-H650 cells. Total RNA was extracted from confluent cultures and analyzed for the presence of IL-4R and GAPDH transcripts by RT-PCR. The amplified products were run on 1% agarose-ethidium bromide gels. Bands at 335 bp (IL-4R) and 1000 bp (GAPDH) were observed. Immunohistochemical determination of IL-4Rα upon permeabilized NCI-H650 cells using (b) rabbit polyclonal anti-human IL-4Rα antibody (c) and non-immune rabbit IgG serum (control). Secondary antibody incubations were performed with Alexa Fluor (568 nm)-labeled mouse antibody. Cells were counterstained with DAPI and photographed using dapi: rhodamine filters at 25:2000 ms exposure. Block arrows in (1b) are indicative of specific staining to IL-4 receptors on cell surface.

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Respiratory Research

ISSN: 1465-993X

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