Figure 6
From: VEGF is upregulated by hypoxia-induced mitogenic factor via the PI-3K/Akt-NF-κB signaling pathway
HIMF activates NF-κB in MLE-12 cells. Cells were co-transfected with pNFκB-luc and pRL-TK, with or without stimulation of HIMF protein for various periods as indicated. (6A) Dual-luciferase assay indicated that MLE-HIMF had higher NF-κB activity than their control counterparts. (6B) Dual-luciferase assay indicated that HIMF protein increased the NF-κB activity in MLE-12 cells in a dose-dependent manner. The symbol (*) indicates a significant increase from MLE-12 parent controls or controls untreated with HIMF (P < 0.05). Triplicate experiments were performed with essentially identical results.