Figure 1
From: VEGF is upregulated by hypoxia-induced mitogenic factor via the PI-3K/Akt-NF-κB signaling pathway
HIMF enhances VEGF expression in mouse lungs. Recombinant HIMF protein was intratracheally instilled into adult mice (200 ng/animal in 40 μl saline, n = 6 for each group). The vehicle controls were instilled with saline (40 μl/animal, n = 3). Six and twenty four hours later, the mouse lungs were collected. (A) The immunohistochemical staining results indicated that HIMF protein instillation resulted in a significant increase of VEGF production, mainly located at alveolar type II (left panels, arrows) and airway epithelial cells (right panels, arrows). Scale bars: 100 μm. (B) Western blot indicated that VEGF expression was enhanced in HIMF-instilled mouse lungs. The symbol (*) indicates a significant increase from control mouse lungs instilled with saline only (P < 0.05).