Figure 1

The isolated CD4⁺and CD8⁺T cells are highly purified. Tonsils were minced and T cells were separated from other mononuclear cells using SRBC rosetting followed by density gradient centrifugation. CD4⁺ and CD8⁺ T cells were further isolated by negative selection using magnetic beads. The isolation procedure was accompanied by FACS analysis of surface phenotype (purity > 95%).