Skip to main content
Figure 13 | Respiratory Research

Figure 13

From: Airway smooth muscle relaxation results from a reduction in the frequency of Ca2+ oscillations induced by a cAMP-mediated inhibition of the IP3 receptor

Figure 13

Effect of the ryanodine on contraction and Ca2+signaling. (A) MCh (200 nM) induced contraction of the same airway in the absence or presence of ryanodine (50 μM, Rya). (B) Changes in fluorescence (upper trace) and frequency of oscillatory Ca2+ signaling (lower trace) of a single airway SMC treated with 200 nM MCh and 50 μM ryanodine. Representative traces of 6 different slices from 4 mice.(C) A fluorescence image of part of the airway obtained by confocal microscopy. The dashed white oval indicates the position and size of the zone of UV illumination (5 s, OD 0.6). L, lumen; ECs, epithelial cells; SMC, smooth muscle cell. Dotted line indicates the interface between ECs and SMCs. Scale bar = 18 μm. Point A was located at the center of the zone of illumination on a single SMC and Point B was located on the same SMC outside the zone of illumination. (D) The local Ca2+ signals at point A and B induced by flash photolysis of caged-Ca2+ in the absence or presence of 50 mM K+ (incubation time of 3 min). Representative traces of 5 different slices from 3 mice.

Back to article page