Table 2 Primer sequences used for qPCR
From: Lung fibroblasts from patients with emphysema show markers of senescence in vitro
HUGO ID | 5'- sense primer- 3' | 5'- antisense primer – 3' | product (bp) | Sequence ID |
|---|---|---|---|---|
PBGD [26] | CACACAGCCTACTTTCCAAGC | TTCAATGTTGCCACCACACT | 155 | NM_000190.2 |
IGFBP-3 | CCTGCCGTAGAGAAATGGAA | GAAGGGCGACACTGCTTT | 127 | NM_000598 |
IGFBP-5 | GAGCAAGTCAAGATCGAGAGAGA | GAAAGTCCCCGTCAACGTA | 463 | M65062 |
IGFBP-rP1 | CTGCGAGCAAGGTCCTTCCATA | CAGGTTGTCCCGGTCACCA | 184 | NM_001553 |
CTGF [22] | CCTGCAGGCTAGAGAAGCAGA | TGCACTTTTTGCCCTTCTTAATGT | 90 | NM_001901 |
Cyr61 | ACACCAAGGGGCTGGAATG | TGGGGACACAGAGGAATG | 193 | AF31385 |
LOXL2 | CGGAGGATGTCGGTGTGGT | GCTTGCGGTAGGTTGAGAGG | 150 | NM_002318 |
OAZ1 | AGGTGGGCGAGGGAATAG | ATGCGTTTGGCGTCTGTG | 150 | U09202 |
FOSL1 | CAGGCGGAGACTGACAAA | GGGAAAGGGAGATACAAGGT | 217 | NM_005438 |
CDK4 | TGCAGTCCACATATGCAACAC | CAGCCCAATCAGGTCAAAG | 137 | M14505 |