Skip to main content

Table 1 siRNA and asDNA used in this study

From: Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo

Name Sequence (from 5' to 3')
Antisense oligonucleotides
FITC-asDNA UACGATGCTGCTAGCUAGUA
LacZ as 1 AUCAUCATTAAAGCGAGUGG
LacZ as 2 AUGGAAACCGTCGATAUUCA
LacZ as 3 GGAAGGATCGACAGAUUUGA
LacZ as 4 AACAGGTATTCGCTGGUCAC
LacZ as 5 CCAUGCCGTGGGTTTCAAUA
Control 1 UACGATGCTGCTAGCUGUAC
Control 2 UCGAUGTAGCTAGCTAUGUC
αENaC asODN 1 GAAUGGAGGAGGATGUCAGA
αENaC asODN 2 ACCGUGGATGGTGGTAUUGU
αENaC asODN 3 GUUGAAACGACAGGTAAAGA
αENaC asODN 4 GUGGAAGATGTGCTGAAGUG
αENaC asODN 5 UUCUGGTTGCACAGTUGGAA
Synthetic small interfering RNAs
LacZ siRNA 1 Sense Z1
Antisense Z1
5'PO4 r(acc cug gcg uua ccc aac uua a)3'OH
5'PO4 r(aag uug ggu aac gcc agg guu u)3'OH
LacZ siRNA 2 Sense Z2
Antisense Z2
5'PO4 r(gcu ggc ugg agu gcg auc uu)3'OH
5'PO4 r(gau cgc acu cca gcc agc uu)3'OH
LacZ siRNA 3 Sense Z3
Antisense Z3
5'PO4r(ccu auc cca uua cgg uca auc c)3'OH
5'PO4r(auu gac cgu aau ggg aua gg)3'OH
LacZ siRNA 4 Sense Z4
Antisense Z4
5'PO4 r(ccg acu aca caa auc agc gau u)3'OH
5'PO4 r(ucg cug auu ugu gua guc ggu u)3'OH
LacZ siRNA 5 Sense Z5
Antisense Z5
5'PO4 r(guu cag aug ugc ggc gag uu)3'OH3
5'PO4 r(cuc gcc gca cau cug aac uu)3'OH
LacZ siRNA 6 Sense Z6
Antisense Z6
5'PO4 r(cuu uaa cgc cgu gcg cug uu)3'OH
5'PO4 r(cag cgc acg gcg uua aag uu)3'OH
LacZ siRNA 7 Sense Z7
Antisense Z7
5'PO4 r(gcc aau auu gaa acc cac gg)3'OH
5'PO4 r(gug ggu uuc aau auu ggc uu)3'OH
LacZ siRNA 8 Sense Z8
Antisense Z8
5'PO4 r(cug ugc cga aau ggu cca uca a)3'OH
5'PO4 r(gau gga cca uuu cgg cac agc c)3'OH
LacZ siRNA 9 Sense Z9
Antisense Z9
5'PO4 r(gca aaa cac cag cag cag uu)3'OH
5'PO4 r(cug cug cug gug uuu ugc uu)3'OH
LacZ siRNA 10 Sense Z10
Antisense Z10
5'PO4 r(gug acc agc gaa uac cug uu)3'OH
5'PO4 r(cag gua uuc gcu ggu cac uu)3'OH
Control siRNAs
CAT sense
antisense
5'PO4 r(gag uga aua cca cga cga uuu c) 3' OH
5'PO4 r(aau cgu cgu ggu auu cac ucc a) 3' OH
FITC-CAT sense
antisense
5'PO4 r(gag uga aua cca cga cga uuu c) 3' Fluorescein
5'PO4 r(aau cgu cgu ggu auu cac ucc a) 3' OH
GFP sense
Antisense
5'PO4 r(gca agc uga ccc uga agu uca u) 3' OH
5'PO4 r(gaa cuu cag ggu cag cuu gcc g) 3' OH