In vitroscreening of ENaC asODN in M1 cells. M1 cells were transfected with 5 different αENaC asODN and a control ODN complexed to Lipofectamine 2000. Forty-eight hours after transfection mRNA was extracted and αENaC mRNA was quantified. Data are presented as mean +/- SEM. ** indicates p < 0.01 and *** = p < 0.001 when compared to control, n = 6/group.