Table 1 Primers used for allele-specific PCR amplification and sequencing.
From: Limited β2-adrenoceptor haplotypes display different agonist mediated airway responses in asthmatics
Primer | Fragment | direction | Sequence |
|---|---|---|---|
2 | +396/+377 | Antisense | 5'-gtagcgatccactgcgatca-3' |
7 | -387/-367 | Sense | 5'-gggccccgcccgggccagcct-3' |
8 | -387/-367 | Sence | 5'-gggccccgcccgggccagccc-3' |
214 | -1081/-1061 | Antisense | 5'-ctgcaaattcctaaggagggc-3' |
215 | -349/-367 | Antisense | 5'-ctcgccctccttctcctga-3' |
216 | -349/-367 | Antisense | 5'-ctcgccctccttctcctgg-3' |