Figure 7
These photographs show typical results. Each density of PCR products was measured using Adobe Photoshop 6.0. G6PD was measured as an internal control. The ratio of each Smad molecule against G6PD is shown by a histogram. bp: base pair. (a) Effects of EM703 on the expression of Smad3 and Smad4 mRNA in lung tissue on day 7 after bleomycin injection in ICR mice (n = 3). #P < 0.05, ##P < 0.01, significantly different from NS-treated group; **P < 0.01, *P < 0.05, significantly different from bleomycin-alone-treated group. The values are means, and the bars are SD. (b) Expression of Smad3 and Smad4 mRNA in MLg2908. TGF-β: 5 ng/ml, EM703: 5 μg/ml. Each group as described in Materials and Methods: Cell cultures for the expression of mRNA and protein assay.