Following damage, the majority of bone marrow-derived airway cells express an epithelial marker

Respiratory Research

Table 1 Contribution of Y chromosome bone marrow SP cells to female recipient tracheas.

Mouse	Y Chromosome +'ve	Total DAPI Nuclei	% Y Chromosome +'ve
CW1	2	2065	0.09
CW2	3	1738	0.17
CW3	2	1818	0.11
CW4	4	2098	0.19
W1	24	2356	1.02
W2	41	3777	1.09
W3	24	2165	1.11
W4	40	2788	1.43
W5	46	2885	1.59
SP1	186	12509	1.49
SP2	0	1281	0
SP3	41	7092	0.58
SP4	80	9660	0.83
SP5	42	4821	0.87

The number of Y chromosome positive cells in the tracheal epithelial lining of female mice irradiated and transplanted with male whole bone marrow (W1-5 and CW1-4) or SP cells (SP1-5) after 3 months. CW1-4 were undamaged animals, and SP1-5 and W1-5 were 1 week post polidocanol damage. Cells are scored by Y chromosome FISH and the number of cells counted using DAPI staining of nuclei.

ISSN: 1465-993X