Figure 3

Effect of pharmacological leukotrienes activity inhibition on lung IL-1 and TNF-α immunostaining. After bleomycin injection in WT mice, positive staining for IL-1 (A) was localized mainly in inflammatory cells and in vascular endothelium. There was a marked reduction in the IL-1 immunostaining in the lungs of MK-571 group (B) and in the lungs of Zileuton group (C). TNF-α was localized mainly in inflammatory cells and in bronchial epithelium of lungs in the bleomycin group (E). A marked reduction in TNF-α immunostaining in lungs of MK-571 (F) and in Zileuton group (G). Original magnification: 150×. This figure is representative of at least 3 experiments performed on different experimental days.