|
Proinflammatory cytokine (pg/ml)
|
Treatment
|
|---|
| |
Control
|
CSE (0.2%)
|
CSE (0.5%)
|
CSE (1.0%)
|
TNF-α (10 ng/ml)
|
|---|
|
Interleukin-8 (IL-8)
|
56.2 ± 7.1
|
126 ± 40.6***
|
171 ± 21.8***
|
418 ± 52.3***
|
591 ± 76.2***
|
|
Interleukin-6 (IL-6)
|
87. 3 ± 7.2
|
187 ± 43.5***
|
275 ± 31.6***
|
476 ± 54.8***
|
623 ± 51.7***
|
- Primary human small airway epithelial cells (SAEC) were treated for 24 hr with cigarette smoke extract (0.2–1.0%) prepared from 1R3F research grade cigarettes and TNF-α was used as a positive control (10 ng/ml). Cells were harvested, and supernatants were collected for the measurement of IL-8 and IL-6 levels by sandwich ELISA. CSE treatment dose-dependently induced the release of both IL-8 and IL-6 from human SAEC. Data represent mean ± SEM of 3 individual experiments. ***p < 0.001 compared to control values. CSE: cigarette smoke extract.
