Figure 5

Representative flow cytometry dot plots showing calcein dye transfer trough gap junctions at different time points in a normal fibroblast line in the absence (A) and in the presence (B) of CBX (100 uM). At time 0 the recipient cells (DiI and calcein negative) are in the left-bottom box and donor cells (DiI and calcein positive) in the right-upper box. Note in A the time-dependent increase in calcein positive cells in the recipient cell populations (right-bottom box). This increase is almost completely blocked by CBX in B.