Figure 2
Selective induction of chemokine mRNA by airway epithelial cultures cross-linked with ICAM-1. (Figure A. A549 cells) (Figure B. NHBE cells). Cultures were incubated with control IgG1 mAb and anti-mouse IgG F(ab')2 (white columns) or anti-ICAM-1 mAb and anti-mouse IgG F(ab')2 (black columns) for the indicated times before RNA was isolated for real time RT-PCR analysis of both RANTES and IL-8 message (40 PCR cycles). Messenger RNA of the 18s ribosomal gene was amplified under the same conditions and was used as the internal control. (n = 6; * = significantly different from control; p < 0.05).