Figure 1
Cross-linking of ICAM-1 induces phosphorylation of MAP kinases in airway epithelial cells. (Figure A. A549 cells) (Figure B. NHBE cells). Lane 1 represents cultures that were cross-linked with control IgG1 mAb and anti-mouse IgG F(ab')2 for 10 min. Lanes 2–5 represent cultures that were cross-linked with anti-ICAM-1 mAb and anti-mouse IgG F(ab')2 for 5 min (lane 2), 10 min (lane 3), 15 min (lane 4), and 30 min (lane 5). Lane 6 represents cultures that were exposed with anti-ICAM-1 mAb alone for 10 min. Lane 7 represents cultures that were exposed with anti-mouse IgG F(ab')2 mAb alone for 10 min. Lane 8 represents cultures that were exposed to anti-VCAM-1 mAb and anti-mouse IgG F(ab')2 for 10 min. Fold increase in amounts of phosphorylated MAP kinase proteins as indicated above are expressed as the mean ± SD in three different experiments.